![]() The developed assay was used to quantitate ACoA and MCoA levels in various tissues of rat.įood is essential for human survival. ![]() ACoA and MCoA were stable in a battery of stability studies viz. The intra- and inter-day precision values for ACoA and MCoA met the acceptance as per FDA guidelines. A linear response function was established for the range of concentrations 1.09-2187 and 1.09-2193 ng/mL for ACoA and MCoA, respectively. The total run time was 3 min and the elution of both analytes (ACoA, MCoA) and IS occurred at 1.28 min this was achieved with a mobile phase consisting of 5 mM ammonium formate (pH 7.5)-acetonitrile (30:70, v/v) delivered at a flow rate of 1 mL/min on a monolithic RP-18e column. Simple acidification followed by dilution using an assay buffer process was used to extract ACoA, MCoA and IS from surrogate matrix and tissue samples. LC-MS/MS was operated under the multiple reaction-monitoring mode using the electrospray ionization technique. A highly sensitive and specific LC-MS/MS method was developed for simultaneous estimation of acetyl co-enzyme A (ACoA) and malonyl co-enzyme A (MCoA) in surrogate matrix using n-propionyl co-enzyme A as an internal standard (IS).
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